Cellulase: Types and Action, Mechanism, and Uses by Adam E. Golan

By Adam E. Golan

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Extra resources for Cellulase: Types and Action, Mechanism, and Uses (Biotechnology in Agriculture, Industry and Medicine)

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Ionata et al. , 1989; Mountford and Orpin, 1994]. , 1997]. , 2002; Ljungdahl, 2008]. The cellulose/hemicellulose degrading system of Piromyces equi, so called because this Fungus was isolated from the caecum of a pony [Orpin, 1981], consists of a large multienzyme complex, which accounts for up to 90% of the cellulase, mannanase and xylanase activities produced by the Fungus. Eberhardt et al. [2000] described the elucidation of the primary structures and enzyme features of two endoglucanases from P.

2003]. The microorganism, identified as Moorella strain F21, secretes cellulolytic enzymes after growth on ball-milled cellulose or Avicel as carbon source, and ferments cellulosic materials to organic acids. 2 Fungal Cellulases Among the thermophilic Fungi, only a few number is described to be cellulase-producer (Table 9). The thermophilic filamentous fungus Humicola sp. , 1997]. The cellulase system of the thermophilic fungus Humicola insolens possesses a battery of enzymes that allows the efficient utilization of cellulose.

In 2002, Lu et al. [2002] reported that C. 8 kDa, as determined by SDS-PAGE. 5 and 60°C, and it retained 30% activity after 60 min at 70°C. , 2006]. The endoglucanases from this Fungus have been recognized as potentially important in denim washing. In fact, the supernatant from M. albomyces worked well in biostoning, with low backstaining. , 2004]. Cel45A was relatively heat stable retaining 70% activity after 1 h at 80°C. 0, respectively. 0. Cel45A has been crystallized [Hirvonen and Papageorgiou, 2002].

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