By Rajyalakshmi Luthra, Rajesh Singh, Keyur Pravinchandra Patel
This up-to-date quantity explores a wide selection of medical purposes of PCR similar to detecting DNA methylation, detection of viruses and protozoa in infectious ailments, estimation of gene replica quantity aberrations, primer extension coupled with mass spectroscopy, and excessive throughput NGS suggestions. the applying of PCR has proven significant price within the learn of genomics and transcriptomics, not just for discovery but additionally for regimen scientific functions, and it kinds the cornerstone of custom-made medication. Written for the hugely winning Methods in Molecular Biology sequence, chapters comprise short introductions to their respective themes, lists of the required fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and pointers on troubleshooting and averting identified pitfalls.
Thorough and authoritative, Clinical purposes of PCR, 3rd Edition serves as a great consultant for researchers aiming to appreciate the foundations in the back of every one program and for his or her implementation within the laboratory.