By Anthony N. Laskovski
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Vimala K, Murali Mohan Y, Samba Sivudua K, Varaprasada K, Ravindra S, Narayana Reddy N, Padma Y, Sreedhar B, MohanaRaju K, Fabrication of porous chitosan films impregnated with silver nanoparticles: A facile approach for superior antibacterial application. Colloids Surf. B: Biointerfaces, 2010; 76: 248-258. Wang CC, Su CH, Chen CC. Water absorbing and antibacterial properties of N-isopropyl acrylamide grafted and collagen/chitosan immobilized polypropylene nonwoven fabric and its application on wound healing enhancement.
2010). For this study human cervical carcinoma cells with stable expression of enhanced green fluorescent protein (EGFP) were prepared. Then the gene-silencing effect of siRNA complexes with chitosans of different molecular weight (20, 45, 200, and 460 kDa) was examined. The best results were obtained with LMWC – 20 kDa. The molecular weight of chitosan did not affect the particle size of the CS/siRNA complex, but higher molecular weights did favour complex formation. 5 Tissue engineering There is also a special place for chitin/chitosan in the field of tissue engineering.
Charoenthai et al. showed that LMWC had better pelletforming properties (diameter, sphericity) and guaranteed drug release control. The effect of the molecular weight of four different samples of chitosan (160, 580, 1350, 1930 kDa) on the efficacy of two antibiotics (tobramycin and ofloxacin) used during external ocular infections was investigated over time by Felt et al. , 2001). The antibiotic content in tears was found to increase when HMWC was employed as compared to the controls and samples with a lower molecular weight of chitosan.