Annual Plant Reviews, Plant Proteomics (Volume 28) by Christine Finnie

By Christine Finnie

The proteome contains all protein species caused by gene expression in a cellphone, organelle, tissue or organism. via definition, proteomics goals to spot and characterise the expression trend, mobile position, task, law, post-translational variations, molecular interactions, 3 dimensional buildings and features of every protein in a organic approach. In plant technology, the variety of proteome reviews is swiftly increasing after the final touch of the Arabidopsis thaliana genome series, and proteome analyses of different very important or rising version structures and crop crops are in development or are being initiated. Proteome research in crops is topic to an identical stumbling blocks and boundaries as in different organisms, however the nature of plant tissues, with their inflexible phone partitions and complicated number of secondary metabolites, signifies that additional demanding situations are concerned that will not be confronted while analysing different organisms. This quantity goals to spotlight the ways that proteome research has been used to probe the complexities of plant biochemistry and body structure. it really is aimed toward researchers in plant biochemistry, genomics, transcriptomics and metabolomics who desire to achieve an updated perception into plant proteomes, the data plant proteomics can yield and the instructions plant proteome learn is taking.

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Electrophoresis, 20, 705–711. , Masson, F. and Rabilloud, T. (2000) Membrane proteomics: use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoretic properties. Electrophoresis, 21, 3329–3344. Schägger, H. and von Jagow, G. (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. , 199, 223–231. P. and Kieselbach, T. (2002) Proteome map of the chloroplast lumen of Arabidopsis thaliana.

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2004) Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Mol. Cell. , 3, 1154–1169. Y. H. (2001) Proteolysis in mixed organic-aqueous solvent systems: applications for peptide mass mapping using mass spectrometry. Anal. , 73, 2682–2685. , Rabilloud, T. and Rossignol, M. (1999a) Large scale characterization of plant plasma membrane proteins. Biochimie, 81, 655–661. , Garin, J. and Rossignol, M. (1999b) Towards the recovery of hydrophobic proteins on two-dimensional electrophoresis gels.

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