By Alisher Touraev, Brian P. Forster, Shri Mohan Jain
The discovery of haploid Datura crops in 1964 initiated nice pleasure in plant breeding and genetics groups. fresh years have witnessed a resurgence in actions specially in constructing protocols, opting for genes and mechanisms and big scale advertisement take up.
The identity of controlling genes has pushed sensible genomic experiences which now hyperlink in with experiences in gene expression, metabolism and adjustments in mobile ultra-structure. worldwide soak up via plant breeders has been no much less amazing and invaluable haploid applied sciences is more and more patent secure. the serious task in haploid examine has additionally led to unforeseen findings with novel applications.
The middle of this ebook is predicated at the overseas symposium on "Haploidy in better vegetation III", (Vienna 2006), which attracted best foreign specialists within the box. different invited contributions were integrated to supply a rounded view of activities.
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Additional resources for Advances in Haploid Production in Higher Plants
Uni-lj-si A. Touraev et al. V. 2009 35 36 B. Bohanec the term gynogenesis will be used in this sense as a substitute for the longer descriptive explanation – haploid induction from un-pollinated ovules, ovaries or flower buds. Historically, the first haploid plants originating from female gametophytes were described in Datura stramonium (Blakeslee et al. 1922). These haploid plants formed spontaneously were subsequently found in other species at low frequencies. A more recent review of spontaneously formed haploid plants is given by Palmer and Keller (2005) citing the occurrence of haploid plants in tobacco, rice, maize, Brassica and barley.
Pearl millet). Direct comparison using the pollen developmental stage is therefore problematic. Microscopic analysis of embryo sacs is tedious and requires a skilful embryologist for interpretation of the results. There are therefore much less data available compared to anther/microspore culture, although the embryo sac developmental stages at the time of inoculation have been determined for a number of species. In sugar beet, flower buds collected 1–3 days before anthesis possessed a mature embryo sac (Ferrant and Bouharmont 1994).
Developmental Stage at Inoculation The pollen developmental stage is one of most important factors in androgenesis. Late uni-cellular microspore is often the most responsive target for re-programming a pathway from gametophytic into sporophytic. There is a general opinion that, in contrast to in vitro androgenesis, it is possible to induce a gynogenic response from ovules over a broad range of developmental stages. Data supporting this opinion are to some extent limited. The stage of ovule development at the time of inoculation has not often been clearly determined.